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Wuxi Donglin Sci & Tech Development Co.,Ltd.
Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit

Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit

Place of Origin : China , Canada
Brand Name : DLdevelop
Certification : CE, ISO9001:2015
MOQ : 48T
Price : Negotiation
Packaging Details : Carton Box
Delivery Time : 1-2 Working Days
Payment Terms : TT
Supply Ability : Abundant Supply
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Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit

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two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA KitReady-to-Use ELISA KIT
Product name:Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
Method:Sandwich
Synonyms:

ZNT3; Zinc Transporter 3

Detection range:78.125-5000pg/mL
Reactivity:SLC30A3
Size:96T/48T
Quality guarantee period:for 12 months
Catalog number:DL-SLC30A3-Hu (traditional)DLR-SLC30A3-Hu (ready-to-use)
Assay length1-4.5Hours1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual
Introduction
ItemStandardTest
Description

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLC30A3 in human tissue homogenates or other biological fluids.

Conform
IdentificationColorimetricPositive
CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120LDetection Solution A 1×12mL
Detection Reagent B 1×120LDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
Instruction manual 1Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC30A3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLC30A3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

After TMB substrate solution is added, only those wells that contain SLC30A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

The concentration of SLC30A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant SLC30A3 and the recovery rates were calculated by comparing the measured value to the expected amount of SLC30A3 in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-10291
EDTA plasma(n=5)81-10191
heparin plasma(n=5)80-8984

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SLC30A3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)82-96%83-98%81-99%93-101%
EDTA plasm



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