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Wuxi Donglin Sci & Tech Development Co.,Ltd.
Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit

Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit

Place of Origin : China , Canada
Brand Name : DLdevelop
Certification : CE, ISO9001:2015
MOQ : 48T
Price : Negotiation
Packaging Details : Carton Box
Delivery Time : 1-2 Working Days
Payment Terms : TT
Supply Ability : Abundant Supply
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Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit

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two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA KitReady-to-Use ELISA KIT
Product name:Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit
Method:Sandwich
Synonyms:

ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1

Detection range:0.156-10ng/mL
Reactivity:PYCARD
Size:96T/48T
Quality guarantee period:for 12 months
Catalog number:DL-PYCARD-Hu (traditional)DLR-PYCARD-Hu (ready-to-use)
Assay length1-4.5Hours1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual
1. Overview
Other names:ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1
Function: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Sequence
MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL
DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI
QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYGKV LTDEQYQAVR
AEPTNPSKMR KLFSFTPAWN WTCKDLLLQA LRESQSYLVE DLERS
2. Features
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PYCARD in human serum, plasma, tissue homogenates or other biological fluids.
DETECTION RANGE
0.156-10ng/mL. The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL.
SENSITIVITY
The minimum detectable dose of PYCARD is typically less than 0.065ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of PYCARD.
No significant cross-reactivity or interference between PYCARD and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between PYCARD and all analogues, therefore, cross reactivity may still exist.
IMPORTANT NOTES
1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.
2. The final experimental results will be closely related to the validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available to obtain accurate results.
3. Kits from different batches may be a little different in detection range, sensitivity and color developing time. Please perform the experiment exactly according to the instruction manual included in your kit. Electronic ones on our website are for reference only.
4. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
5. Protect all reagents from strong light during storage and incubation. All bottle caps of reagents should be closed tightly t


























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